The Tooth Fairy is a magical fairy who goes around and collects people's loose teeth from beneath their pillows as seen in the episode You Can't Handle the Tooth!. She is voiced by Fred Tatasciore.
The Tooth Fairy looks like a man but is referred to with pronouns like she and her, she has black hair and wears eyelashes and red lipstick, she also has a big nose and a chiseled chin. She wears a black ripped tank top with cyan underpants and lavender leg warmers. She wears a gold crown with three separate points, she has big white wings, has red fingernails and has a muscular build with hairy arms and legs, she also carries a gold staff with a tooth on it. When wearing her new corset, she's wearing teeth arranged to treat for her sciatica and she's no longer wearing her black tank top.
The Tooth Fair is very enthusiastic about collecting teeth as she's been doing it for hundreds of years, she believes that the bigger the teeth the better. Before collecting someone's teeth, she has to gargle mouthwash and before she leaves, she leaves a pile of dandruff and some feathers behind. She has a sciatica problem and was shown to be comply with Uncle Grandpa when he offer to solve her problem if she decided to let them free and forgive Pizza Steve.
- The Tooth Fairy may be the first character on Uncle Grandpa to be transsexual.